NAST Molecular Biotechnology Unit (previously called Biotechnology Unit) started researching at DNA level since 2002 with molecular diagnosis of Citrus Huanglongbing (HLB) disease. Prior to this, it was engaged in other biotechnological research activities such as production of disease free planting materials of Citrus through Shoot Tip Grafting (STG) in vitro tissue culture technique, Bacillus thuringiensis based biological pesticide, Citrus Tristeza Virus (CTV) diagnosis using ELISA technique and plant propagation using various plant growth regulators etc. Nepal Academy of Science and Technology (NAST) is an autonomous apex body established in 1982 to promote science and technology in the country. The Academy is entrusted with four major objectives: advancement of science and technology for all-round development of the nation; preservation and further modernization of indigenous technologies; promotion of research in science and technology; and identification and facilitation of appropriate technology transfer. - See more at: http://nast.gov.np/new/index1.php?option=e6r5wlVM8od_u8Y0CdwsDiTfg0cohLLpEcNS8hphu-0&id=Bl0QqdhY8KY50uI1NrtvmFwc0gKQyB1ysIRqEd_qdnU#sthash.ukXjubZe.dpuf